Journal: Endocrinology

Article Title: Short-Term High-Fat Diet Increases Leptin Activation of CART Neurons and Advances Puberty in Female Mice

doi: 10.1210/en.2017-00452

Figure Lengend Snippet: Representative photomicrographs (magnification ×63) showing immunostaining for (A) NKB in red and (B) DYN in blue and Kiss1-hrGFP neurons in green, and the overlapping images of the rostral arcuate of female mice (PN32) fed an RD or HFD. The images are the maximum intensity projection of a series of 35 individual optical planes. Scale bar = 20 μm.

Article Snippet: The sections were washed, and, after blocking [5% normal horse serum, 0.4% Triton X-100 in tris(hydroxymethyl)aminomethane; pH 7.6], the slices were incubated overnight in different primary antibody combinations: rabbit anti-NKB antibody (1:2500; Bachem, Torrance, CA), guinea pig anti-DYN antibody (1:250; Neuromics, Minneapolis, MN) or rabbit anti–p-STAT3 (1:1000; Cell Signaling Technology, Danvers, MA), and goat anti-CART (1:500; Santa Cruz Biotechnology, Dallas, TX).

Techniques: Immunostaining

Journal: Endocrinology

Article Title: Short-Term High-Fat Diet Increases Leptin Activation of CART Neurons and Advances Puberty in Female Mice

doi: 10.1210/en.2017-00452

Figure Lengend Snippet: Antibody Table

Article Snippet: The sections were washed, and, after blocking [5% normal horse serum, 0.4% Triton X-100 in tris(hydroxymethyl)aminomethane; pH 7.6], the slices were incubated overnight in different primary antibody combinations: rabbit anti-NKB antibody (1:2500; Bachem, Torrance, CA), guinea pig anti-DYN antibody (1:250; Neuromics, Minneapolis, MN) or rabbit anti–p-STAT3 (1:1000; Cell Signaling Technology, Danvers, MA), and goat anti-CART (1:500; Santa Cruz Biotechnology, Dallas, TX).

Techniques: Sequencing